BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//Genetic Engineering and Society Center - ECPv6.15.20//NONSGML v1.0//EN
CALSCALE:GREGORIAN
METHOD:PUBLISH
X-WR-CALNAME:Genetic Engineering and Society Center
X-ORIGINAL-URL:https://ges.research.ncsu.edu
X-WR-CALDESC:Events for Genetic Engineering and Society Center
REFRESH-INTERVAL;VALUE=DURATION:PT1H
X-Robots-Tag:noindex
X-PUBLISHED-TTL:PT1H
BEGIN:VTIMEZONE
TZID:America/New_York
BEGIN:DAYLIGHT
TZOFFSETFROM:-0500
TZOFFSETTO:-0400
TZNAME:EDT
DTSTART:20220313T070000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:-0400
TZOFFSETTO:-0500
TZNAME:EST
DTSTART:20221106T060000
END:STANDARD
BEGIN:DAYLIGHT
TZOFFSETFROM:-0500
TZOFFSETTO:-0400
TZNAME:EDT
DTSTART:20230312T070000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:-0400
TZOFFSETTO:-0500
TZNAME:EST
DTSTART:20231105T060000
END:STANDARD
BEGIN:DAYLIGHT
TZOFFSETFROM:-0500
TZOFFSETTO:-0400
TZNAME:EDT
DTSTART:20240310T070000
END:DAYLIGHT
BEGIN:STANDARD
TZOFFSETFROM:-0400
TZOFFSETTO:-0500
TZNAME:EST
DTSTART:20241103T060000
END:STANDARD
END:VTIMEZONE
BEGIN:VEVENT
DTSTART;TZID=America/New_York:20231003T120000
DTEND;TZID=America/New_York:20231003T130000
DTSTAMP:20260504T092520
CREATED:20230814T180537Z
LAST-MODIFIED:20231005T174831Z
UID:10000407-1696334400-1696338000@ges.research.ncsu.edu
SUMMARY:Amarish Yadav—CRISPR/Cas-9-Based Gene Drive To Suppress Agricultural Pests | GES Colloquium
DESCRIPTION:Colloquium Home | Zoom Registration | GES Video Library (current) | Video Archives | Podcast | @GESCenterNCSU | Newsletter \nCRISPR/Cas-9-Based Gene Drive To Suppress Agricultural Pests \nAmarish Yadav\, PhD\, Postdoctoral Fellow (Max Scott Lab) at NC State | Profile | Google Scholar \nA discussion about the molecular genetics of homing gene drives disrupting doublesex gene\, as well as its potential and challenges in the D. suzukii population control. \nDownload seminar poster\nAbstract\nThe CRISPR/Cas9-based homing ‘gene drive’ has emerged as a revolutionary genetic-based method that holds great promise for control of insect pests. Insect pests pose a significant risk to global crop loss\, food security\, and public health. In recent years\, considerable progress has been made in the field of modern gene-drive which advances our understanding of its genetic and molecular mechanisms\, biocontainment strategies\, potential risks\, and challenges. Using the genome-editing tool CRISPR/Cas9\, Amarish has successfully developed and evaluated a split (biosafe) homing gene-drive which disrupts doublesex gene for the population control of an agricultural pest\, Drosophila suzukii. The initial homing gene drive strains he made showed dominant female sterility and biased inheritance of up to 70%. The drive construct was modified such that females were dominant fertile (recessive sterile) and the Cas9 construct re-engineered using D. suzukii components. The final split-homing gene-drive strains showed 94-99% biased inheritance of the engineered genetic element and recessive female sterility. \nIn his talk\, Amarish will discuss the molecular genetics of the homing gene drives disrupting doublesex gene\, as well as its potential and challenges in the D. suzukii population suppression. \nRelated links: \n\nYadav\, A. K.\, Butler\, C.\, Yamamoto\, A.\, Patil\, A. A.\, Lloyd\, A. L.\, & Scott\, M. J. (2023). CRISPR/Cas9-based split homing gene drive targeting doublesex for population suppression of the global fruit pest Drosophila suzukii. Proceedings of the National Academy of Sciences\, 120(25)\, e2301525120. https://doi.org/10.1073/pnas.2301525120. PDF (requires Unity ID)\nDownload PowerPoint slide deck\n\nSpeaker Bio\nDr. Amarish Yadav is a postdoctoral research scholar in Prof. Max Scott’s laboratory at NC State University\, where he has been working on the development of genetic-based pest control methods such as homing gene drives and evaluating safeguards in the agricultural pest spotted wing drosophila (Drosophila suzukii). His doctoral research at Banaras Hindu University in India was to investigate the genetic and molecular aspects of cancer progression linked to the loss of cell-polarity regulators function in Drosophila melanogaster. During his postdoctoral research at NC State\, using CRISPR/Cas9 technology\, he developed the first split homing gene drive in spotted wing drosophila which targets doublesex\, a gene essential for female fly development. In addition\, he has generated various transgenics and eye-color mutants to be useful in the D. suzukii genetics research. Amarish is currently assessing the population-suppression potential of gene drive strains at laboratory scale as well as the influence of different genetic backgrounds on the gene drive efficiency in this pest. \n\nGES Colloquium is jointly taught by Drs. Jen Baltzegar and Dawn Rodriguez-Ward\, who you may contact with any class-specific questions. Colloquium will be held in person in the 1911 Building\, room 129\, and live-streamed via Zoom. \nPlease subscribe to the GES newsletter and Twitter for updates.
URL:https://ges.research.ncsu.edu/event/colloquium-2023-10-03/
LOCATION:1911 Building\, Room 129 (North Campus)\, 10 Current Dr.\, Raleigh\, NC\, United States
CATEGORIES:Colloquium,GES Event
ATTACH;FMTTYPE=image/jpeg:https://ges.research.ncsu.edu/wp-content/uploads/2023/09/20231003_Amarish-Yadav_Colloquium_960x540.jpg
ORGANIZER;CN="GES Center":MAILTO:gesocietycenter@ncsu.edu
END:VEVENT
END:VCALENDAR